CCL12/MCP-5 Antibody (63205) [Unconjugated]

Recombinant Mouse CCL12/MCP‑5 (Catalog # 428‑P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB428). The ND50 is typically 0.5‑2.5 µg/mL. Maximum neutralization observed was around 80%.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 63205
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL12/MCP-5 Antibody (63205) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL12/MCP-5
  Gly23-Gly104
  Accession # Q62401.1
• Specificity: Detects mouse CCL12/MCP‑5 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccl12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse CCL12/MCP-5 Biotinylated Antibody (Catalog number BAF428)
  Standard: Recombinant Mouse CCL12/MCP-5 (Catalog number 428-P5)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL12/MCP-5 Antibody (63205) [Unconjugated]

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL12/MCP-5 Antibody (MAB428)(1)

Publication using MAB428

• Arivazhagan Roshini, Chandra Goparaju, Somanath Kundu, Mohan S. Nandhu, Sharon L. Longo, John A. Longo et al. The extracellular matrix protein fibulin-3/EFEMP1 promotes pleural mesothelioma growth by activation of PI3K/Akt signaling Frontiers in Oncology 2022-10-11 [PMID: 36303838]

Bioinformatics

• Gene Symbol: Ccl12
• Uniprot:
  • Q62401.1()