CCL12/MCP-5 Antibody (63205) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap), ELISA(Det)
• Clone: 63205
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

CCL12/MCP-5 Antibody (63205) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse CCL12/MCP-5
  Gly23-Gly104
  Accession # Q62401.1
• Specificity: Detects mouse CCL12/MCP‑5 in ELISAs and Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL12/MCP-5 Antibody (63205) [Alexa Fluor® 405]

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid (aa) sequence identity in the mature protein). Mouse CCL12 encodes a 104 aa residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR2 but not CCR1, CCR3, or CCR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.