CCDC8 Antibody

Western Blot: CCDC8 Antibody [NBP1-50021] - Detection of Human CCDC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated CCDC8, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CCDC8 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 350 and 400 of human Coiled-Coil Domain Containing 8 using the numbering given in entry NP_114429.2 (GeneID 83987).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCDC8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCDC8 Antibody

• coiled-coil domain containing 8
• coiled-coil domain-containing protein 8
• DKFZp564K0322

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCDC8 Antibody (NBP1-50021)(2)

Publications using NBP1-50021

• Hanson D, Stevens A, Murray PG et al. Identifying biological pathways that underlie primordial short stature using network analysis. J. Mol. Endocrinol. 2014-04-07 [PMID: 24711643] (WB, IP, Human)
• Dai C, Tang Y, Jung SY et al. Differential effects on p53-mediated cell cycle arrest vs. apoptosis by p90. Proc Natl Acad Sci U S A 2011-11-01 [PMID: 22084066]

Bioinformatics

• Gene Symbol: CCDC8
• Entrez:
  • 83987(Human)
• Uniprot:
  • Q9H0W5(Human)