CCDC134 Antibody (815606)

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCDC134 Monoclonal Antibody (Catalog # MAB7786) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCDC134 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 815606
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCDC134 Antibody (815606) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CCDC134
  Ala23-Leu229
  Accession # Q8C7V8
• Specificity: Detects mouse CCDC134 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human CCDC134 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCDC134
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCDC134 Antibody (815606)

• CCDC134
• coiled-coil domain containing 134
• coiled-coil domain-containing protein 134
• dJ821D11.3
• FLJ22349
• MGC21013

Background

Coiled-coil domain containing 134 (CCDC134) is a widely expressed glycoprotein that is secreted as a 38 kDa molecule. CCDC134 overexpression inhibits kinase activity in the Raf-1/MEK/ERK and JNK/SAPK pathways. In humans, CCDC134 polymorphisms are associated with reduced levels of Amyloid beta peptide (1-42) in the cerebrospinal fluid, a potential biomarker for Alzheimer’s disease. Alternative splicing of mouse CCDC134 generates a short isoform that lacks the N-terminal 37 amino acids including the signal peptide. Mouse CCDC134 shares 90% and 95% aa sequence identity with human and rat CCDC134, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCDC134
• Uniprot:
  • Q8C7V8()