CCAR1 Antibody

Images

 
Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human DIS on HeLa whole cell lystate using NB100-258. Controls: Normal IgG or another rabbit anti-DIS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

CCAR1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in entry NP_060707.2 (GeneID 55749).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCAR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
By Western blot, this antibody recognizes bands around 150 kDa representing DIS. Also useful for Immunoprecipitation.
Positive Control
HeLa Lysate (NB800-PC9)
CCAR1 Lysate (NBL1-08757)
Publications
Read Publication using
NB100-258 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCAR1 Antibody

  • CARP-1
  • CARP1MGC44628
  • Cell cycle and apoptosis regulatory protein 1
  • cell division cycle and apoptosis regulator 1
  • cell division cycle and apoptosis regulator protein 1
  • Death inducer with SAP domain
  • DIS
  • FLJ10590
  • RP11-437A18.1

Background

CCAR1 was identified as a perinuclear phosphoprotein that regulates apoptosis signals in response to chemotherapeutic agents. Induction of apoptosis by CCAR1 involves the epidermal growth factor receptor (EGFR), mitogen-activated protein kinase pathway and caspase-9 activation. CARP-1 has also been demonstrated as a suppressor of human breast cancer growth. CCAR1 is also known as cell division cycle and apoptosis regulator protein 1, cell cycle and apoptosis regulatory protein 1, CARP-1, death inducer with SAP domain, DIS, MGC44628, and RP11-437A18.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCAR1 Antibody (NB100-258)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCAR1
Entrez
Uniprot