CCAR1 Antibody - BSA Free

Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCAR1 antibody NB100-258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human DIS on HeLa whole cell lystate using NB100-258. Controls: Normal IgG or another rabbit anti-DIS.

Product Discontinued

CCAR1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in entry NP_060707.2 (GeneID 55749).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: By Western blot, this antibody recognizes bands around 150 kDa representing DIS. Also useful for Immunoprecipitation.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCAR1 Antibody - BSA Free

• CARP-1
• CARP1MGC44628
• Cell cycle and apoptosis regulatory protein 1
• cell division cycle and apoptosis regulator 1
• cell division cycle and apoptosis regulator protein 1
• Death inducer with SAP domain
• DIS
• FLJ10590
• RP11-437A18.1

Background

CCAR1 was identified as a perinuclear phosphoprotein that regulates apoptosis signals in response to chemotherapeutic agents. Induction of apoptosis by CCAR1 involves the epidermal growth factor receptor (EGFR), mitogen-activated protein kinase pathway and caspase-9 activation. CARP-1 has also been demonstrated as a suppressor of human breast cancer growth. CCAR1 is also known as cell division cycle and apoptosis regulator protein 1, cell cycle and apoptosis regulatory protein 1, CARP-1, death inducer with SAP domain, DIS, MGC44628, and RP11-437A18.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCAR1 Antibody (NB100-258)(1)

Publication using NB100-258

• Zhu X, Wang Y, Pi W et al. NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human gamma-globin gene. PLoS One. 2012-10-10 [PMID: 23071749] (WB, Human)

Bioinformatics

• Gene Symbol: CCAR1
• Entrez:
  • 55749(Human)
• Uniprot:
  • NP_060707.2(Human)