CBX1 Antibody (4E12) - Azide and BSA Free

Western Blot: CBX1 Antibody (4E12) [H00010951-M01] - Analysis of CBX1 expression in transfected 293T cell line by CBX1 monoclonal antibody (M01), clone 4E12.Lane 1: CBX1 transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CBX1 Antibody (4E12) [H00010951-M01] - Analysis of monoclonal antibody to CBX1 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: CBX1 Antibody (4E12) [H00010951-M01] - Detection limit for recombinant GST tagged CBX1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CBX1 Antibody (4E12) - Azide and BSA Free Summary

• Immunogen: CBX1 (NP_006798.1, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GKKQNKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESK
• Specificity: CBX1 - chromobox homolog 1 (HP1 beta homolog Drosophila ) (4E12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CBX1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CBX1 Antibody (4E12) - Azide and BSA Free

• CBXM31HP1-BETA
• chromobox homolog 1 (Drosophila HP1 beta)
• chromobox homolog 1 (HP1 beta homolog Drosophila )
• chromobox homolog 1
• chromobox protein homolog 1
• Heterochromatin protein 1 homolog beta
• heterochromatin protein 1-beta
• heterochromatin protein p25 beta
• Heterochromatin protein p25
• HP1 beta homolog
• HP1 beta
• HP1Hsbeta
• HP1Hs-beta
• MOD1
• Modifier 1 protein
• p25beta

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBX1
• Entrez:
  • 10951(Human)
• Uniprot:
  • NP_006798(Human)