CBL [p Tyr731] Antibody (EP973Y)

Product Discontinued

CBL [p Tyr731] Antibody (EP973Y) Summary

• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr731 of human Cbl was used as immunogen.
• Modification: p Tyr731
• Specificity: The antibody detects Cbl phosphorylated on Tyrosine 731.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CBL
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-2000
• Application Notes: This product is useful for: Western Blot, Immunoprecipitation. In Western blot this antibody detects a band at approximately 120kDa.
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with: Mouse, Rat, Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for CBL [p Tyr731] Antibody (EP973Y)

• Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Casitas B-lineage lymphoma proto-oncogene
• CBL
• CBL2
• CBL2E3 ubiquitin-protein ligase CBL
• EC 6.3.2
• EC 6.3.2.-
• oncogene CBL2
• Proto-oncogene c-Cbl
• RING finger protein 55
• RNF55
• RNF55C-CBL
• Signal transduction protein CBL

Background

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBL
• Entrez:
  • 867(Human)
  • 12402(Mouse)
  • 500985(Rat)
• Uniprot:
  • P22681(Human)