Cav1.2 Antibody

Western Blot: Cav1.2 Antibody [NBP1-20896] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cav1.2 Antibody Summary

• Description: Novus Biologicals Goat Cav1.2 Antibody (NBP1-20896) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cav1.2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RARGRPSEEELQD, from the C Terminus of the protein sequence according to NP_000710.5; NP_955630.2; NP_001161097.1; NP_001123299.1; NP_001123301.1; NP_001161096.1; NP_001123302.1; NP_001123303.1 and more.
• Epitope: C terminus
• Specificity: This antibody is expected to recognise all reported human isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CACNA1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1:100-1:2000
• Application Notes: Approx. 200kDa band observed in Human Heart lysate (calculated MW of 240kDa according to NP_000710.5).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cav1.2 Antibody

• alpha-1 subunit
• CACH2
• CACN2
• CACNA1C
• CACNL1A1
• calcium channel, voltage-dependent, L type, alpha 1C subunit
• cardic dihydropyridine-sensitive, alpha-1 subunit
• Cav1.2
• CCHL1A1
• L type, alpha 1 polypeptide, isoform 1, cardic muscle
• L type, alpha-1 polypeptide, isoform 1, cardiac muscle
• splice variant 10*

Background

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cav1.2 Antibody (NBP1-20896)(1)

Publication using NBP1-20896

• Saud ZA, Minobe E, Wang WY et al. Calpastatin binds to a calmodulin-binding site of cardiac Cav1.2 Ca2+ channels. Biochem Biophys Res Commun 2007-12-14 [PMID: 17950697]

Bioinformatics

• Gene Symbol: CACNA1C
• Entrez:
  • 775(Human)
• Uniprot:
  • NP_000710.5(Human)