Cathepsin B Antibody (173317)

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 173317
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Cathepsin B Antibody (173317) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
  His18-Phe339
  Accession # P10605
• Specificity: Detects the pro and active forms of human and mouse Cathepsin B in direct ELISAs and Western blots. Does not cross‑react with recombinant human Cathepsin C, L, L2, O, S, Z, recombinant mouse Cathepsin C, H, L, or Z.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CTSB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin B Antibody (173317)

• APP secretase
• APPS
• Cathepsin B
• Cathepsin B1
• CPSBamyloid precursor protein secretase
• CTSB
• cysteine protease
• EC 3.4.22
• EC 3.4.22.1

Background

Cathepsin B is a cysteine protease that is known to process a number of proteins. It is up-regulated or redistributed in human and animal tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin B Antibody (MAB965)(6)

Publications using MAB965

• Teneale A. Stewart, Katherine Hughes, Alexander J. Stevenson, Natascia Marino, Adler L. Ju, Michael Morehead et al. Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution Journal of Cell Science 5/12/2020 [PMID: 33262312]
• Teneale A. Stewart, Katherine Hughes, Alexander J. Stevenson, Natascia Marino, Adler L. Ju, Michael Morehead et al. Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution Journal of Cell Science 2020-05-12 [PMID: 33262312] (Western Blot, Mouse)
• Cristina Alarcón-Vila, Alberto Baroja-Mazo, Carlos de Torre-Minguela, Carlos M Martínez, Juan J Martínez-García, Helios Martínez-Banaclocha et al. CD14 release induced by P2X7 receptor restricts inflammation and increases survival during sepsis eLife 11/2/2020 [PMID: 33135636]
• Q Shao, M Yang, C Liang, L Ma, W Zhang, Z Jiang, J Luo, JK Lee, C Liang, JF Chen C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis Autophagy, 2019-12-26;0(0):1-16. 2019-12-26 [PMID: 31847700] (ICC, Mouse)
• H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells Oncol Lett, 2017-04-05;13(6):4224-4230. 2017-04-05 [PMID: 28599423] (Western Blot, Human)
• Wendt W, Schulten R, Stichel CC, Lubbert H Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. J. Neurochem., 2009-07-17;110(6):1931-41. 2009-07-17 [PMID: 19627446] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CTSB
• Uniprot:
  • P10605()