Caspase-8 Activity Assay Kit (Colorimetric)

• Reactivity: All-NA
• Applications: Flow
• Suitable Sample Type: Cell and tissue samples

Caspase-8 Activity Assay Kit (Colorimetric) Summary

• Description: Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm.
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP8

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Detection Instrument: Spectrophotometer Assay Time: 4-6 hours

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-8 Activity Assay Kit (Colorimetric)

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

FAQs for Caspase-8 Kit (NBP3-50233). (Showing 1 - 1 of 1 FAQ).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8