Mouse Caspase-8 ELISA Kit (Colorimetric)

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Mouse Caspase-8 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Mouse Caspase-8 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse CASP8 in samples. No significant cross-reactivity or interference between Mouse CASP8 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.05%
Intra-Assay
CV% < 5.32%
Spike Recovery
85-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CASP8

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Wash Buffer (25×)
  3. Concentrated HRP Conjugate (100×)
  4. Certificate of Analysis
  5. Concentrated Biotinylated Detection Ab (100×)
  6. Product Description
  7. Plate Sealer
  8. Reference Standard
  9. Reference Standard & Sample Diluent
  10. HRP Conjugate Diluent
  11. Micro ELISA Plate(Dismountable)
  12. Substrate Reagent
  13. Stop Solution

Alternate Names for Mouse Caspase-8 ELISA Kit (Colorimetric)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 ELISA Kit (NBP2-75040). (Showing 1 - 1 of 1 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP8