Recombinant Human Caspase-7 Protein

Product Discontinued

Recombinant Human Caspase-7 Protein Summary

• Description: Recombinant protein for Human Caspase-7
  
  

  Source:E. coli.
  Amino Acid Sequence:(P55210)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CASP7
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit of the recombinant caspase-7 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
  Specific activity: > 25000 units/mg

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized.
• Concentration: Lyoph
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1 unit per ul in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase-7 Protein

• apoptosis-related cysteine protease
• Apoptotic protease Mch-3
• CASP7
• CASP-7
• caspase 7, apoptosis-related cysteine peptidase
• Caspase7
• Caspase-7
• EC 3.4.22
• EC 3.4.22.60
• ICE-like apoptotic protease 3
• Lice2 alpha/beta/gamma
• Mch3

Background

Caspase-7 (also know as Mch3, ICE-LAP3, CMH-1) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-7 also exists in cells as an inactive proenzyme. During apoptosis procaspase-7 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-7 consists of large and small subunits which associate to form the active enzyme. Active caspase-7 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and genomic maintenance. The recombinant active human caspase-7 was expressed in E. coli. The active caspase-7 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP7