Rat Caspase-7 ELISA Kit (Colorimetric)

ELISA: Rat Caspase-7 ELISA Kit (Colorimetric) [NBP2-75039] - Samples were spiked with high concentrations of Rat Caspase-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Caspase-7 ELISA Kit (Colorimetric) [NBP2-75039] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Rat Caspase-7 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat CASP7 in samples. No significant cross-reactivity or interference between Rat CASP7 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.42%
• Intra-Assay: CV% < 4.96%
• Spike Recovery: 87-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CASP7

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Caspase-7 ELISA Kit (Colorimetric)

• apoptosis-related cysteine protease
• Apoptotic protease Mch-3
• CASP7
• CASP-7
• caspase 7, apoptosis-related cysteine peptidase
• Caspase7
• Caspase-7
• EC 3.4.22
• EC 3.4.22.60
• ICE-like apoptotic protease 3
• Lice2 alpha/beta/gamma
• Mch3

Background

Caspase 7 (CASP7) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 7 is an executioner protein of apoptosis. It is activated upon cell death stimuli and induces apoptosis.

Caspase 7 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 7 form.

Caspase 7 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Caspase-7 ELISA Kit (NBP2-75039)(1)

Publication using NBP2-75039

• El-Sisi AEE, Sokar SS, Shebl AM et al. Octreotide and melatonin alleviate inflammasome-induced pyroptosis through inhibiting TLR4-NF-kappa B-NLRP3 pathway in hepatic ischemia/reperfusion injury Toxicol Appl Pharmacol 2020-11-29 [PMID: 33264646] (ELISA, Rat)

FAQs for Caspase-7 ELISA Kit (NBP2-75039). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CASP7