Caspase-7 Antibody

Images

 
Western Blot: Caspase-7 Antibody [NBP1-19794] - Extracts from PC12 cells.
Immunohistochemistry: Caspase-7 Antibody [NBP1-19794] - Human breast carcinoma tissue.
Western Blot: Caspase-7 Antibody [NBP1-19794] - Extracts from HT-29cells.

Product Details

Summary
Product Discontinued
View other related Caspase-7 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19794
    • Availability
      Product Discontinued

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Caspase-7 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human Caspase 7.
Clonality
Polyclonal
Host
Rabbit
Gene
CASP7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Caspase-7 (M45) pAb in extracts from PC12 cells.
Immunohistochemistry (IHC) analyzes of Caspase-7 (M45) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-7 Antibody

  • apoptosis-related cysteine protease
  • Apoptotic protease Mch-3
  • CASP7
  • CASP-7
  • caspase 7, apoptosis-related cysteine peptidase
  • Caspase7
  • Caspase-7
  • EC 3.4.22
  • EC 3.4.22.60
  • ICE-like apoptotic protease 3
  • Lice2 alpha/beta/gamma
  • Mch3

Background

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Caspase-7 Antibody (NBP1-19794)

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Blogs on Caspase-7.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP7
Entrez