Caspase-7 Antibody - (active/cleaved)

Product Discontinued

Caspase-7 Antibody - (active/cleaved) Summary

• Immunogen: Recombinant catalytically active human Caspase-7 protein was used as immunogen.
• Specificity: This polyclonal antisera recognizes the large (~17-22 kDa) and small (~10 kDa) subunits of active/cleaved caspase-7. Whereas the antisera has a strong preference for active/cleaved caspase-7, in some cell or tissue systems or techniques the antisera may also recognize the proform of Caspase-7 (~35 kDa).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP7
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Application Notes: IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
• Control:

  Caspase-7 Overexpression Lysate

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-7 Antibody - (active/cleaved)

• apoptosis-related cysteine protease
• Apoptotic protease Mch-3
• CASP7
• CASP-7
• caspase 7, apoptosis-related cysteine peptidase
• Caspase7
• Caspase-7
• EC 3.4.22
• EC 3.4.22.60
• ICE-like apoptotic protease 3
• Lice2 alpha/beta/gamma
• Mch3

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-7 polyclonal antisera recognize the large (approx. 17-22 kDa) and small (approx. 10 kDa) subunits of active/cleaved caspase-7. Whereas the antisera have a strong preference for active/cleaved caspase-7, in some cell or tissue systems or techniques the antisera may also recognize the proform of Caspase-7 (approx. 35 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-7 Antibody (NB100-56115)(4)

Publications using NB100-56115

• Chiu CF, Lin YQ, Park JM et al. The novel camptothecin derivative, CPT211, induces cell cycle arrest and apoptosis in models of human breast cancer Biomed. Pharmacother. 2020-08-01 [PMID: 32505820]
• Cevatemre B, Erk?sa M, Aztopal N et al. A promising natural product, pristimerin, results in cytotoxicity against breast cancer stem cells in vitro and xenografts in vivo through apoptosis and an incomplete autopaghy in breast cancer. Pharmacol Res 2017-12-01 [PMID: 29197639] (Mouse)
• Lubet RA, Scheiman JM, Bode A et al. Prevention of chemically induced urinary bladder cancers by naproxen: protocols to reduce gastric toxicity in humans do not alter preventive efficacy Cancer Prev Res (Phila) 2015-04-01 [PMID: 25762530] (IHC-P, Rat)
• Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (WB, IHC-P, Canine, Rat, Mouse)
  
  Details:
  Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne

Bioinformatics

• Gene Symbol: CASP7