Caspase 3/7 Apoptosis Kit

• Reactivity: All-NA
• Applications: Flow
• Suitable Sample Type: Cell samples

Caspase 3/7 Apoptosis Kit Summary

• Description: The Caspase 3/7 Substrates (Green) are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. DAPI co-staining of cells with Caspase 3/7 Substrates allows simultaneous differentiation of late-apoptotic cells, necrotic cells, and caspase 3/7 enzyme activity during different apoptotic periods.
• Kit Type: Apoptosis Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Detection Instrument: Flow Cytometer;Fluorescence Microscope Assay Time: 0.5 hour

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Alternate Names for Caspase 3/7 Apoptosis Kit

• CASP-3
• CASP-7
• caspase-3
• caspase-7
• CPP32
• CPP32B
• ICE-like apoptotic protease 3
• SCA-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.