Caspase 3/7 Apoptosis Kit

• Reactivity: All-NA
• Applications: Flow
• Suitable Sample Type: Cell samples

Caspase 3/7 Apoptosis Kit Summary

• Description: The Caspase 3/7 Substrate (Green) are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy. Caspase 3/7 and Annexin V Double Staining Apoptosis Kit includes Caspase 3/7 Substrates(Green Annexin V-APC Reagent, enables the user to detect two important apoptosis events, caspase 3/7 activation and phosphatidylserine (PS) translocation.
• Kit Type: Apoptosis Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Detection Instrument: Flow Cytometer Assay Time: 0.5 hour

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Alternate Names for Caspase 3/7 Apoptosis Kit

• CASP-3
• CASP-7
• caspase-3
• caspase-7
• CPP32
• CPP32B
• ICE-like apoptotic protease 3
• SCA-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.