Caspase 3/7 Activity Assay Kit (Colorimetric)

• Reactivity: All-NA
• Applications: Flow
• Suitable Sample Type: Cell and tissue samples

Caspase 3/7 Activity Assay Kit (Colorimetric) Summary

• Description: Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.
• Kit Type: Activity Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Detection Instrument: Spectrophotometer Assay Time: 4-6 hours

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase 3/7 Activity Assay Kit (Colorimetric)

• CASP-3
• CASP-7
• caspase-3
• caspase-7
• CPP32
• CPP32B
• ICE-like apoptotic protease 3
• SCA-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.