Caspase-14 Antibody (70A1426) - BSA Free

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of Caspase 14 in mouse spleen lysate using Caspase 14 antibody at 1:1000. Novus's goat anti-mouse Ig HRP secondary antibody (Novus, 20101) and PicoTect ECL substrate solution (Novus, 10087K) were used for this test.

Flow Cytometry: Caspase-14 Antibody (70A1426) [NB100-56718] - Intracellular analysis of Caspase-14 in 10^6 Jurkat cells probed with 1:500 dilution of Caspase-14 antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents Caspase-14 antibody. This antibody was used for this test, and an anti-mouse IgG FITC conjugated secondary antibody.

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of human breast cancer tissue using Caspase 14 antibody at a concentration of 0.1 ug/ml.

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis for Caspase-14 using NB100-56718 at 2 ug/ml dilution against 10 ug of mouse spleen cell lysate.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: 70A1426
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Caspase-14 Antibody (70A1426) - BSA Free Summary

• Description: Novus Biologicals Mouse Caspase-14 Antibody (70A1426) - BSA Free (NB100-56718) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-Caspase-14 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acids 2-18 of mouse Caspase-14.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP14
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry (Intracellular): 1:500/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 18520823)
  • Western Blot 0.1-4 ug/ml
• Application Notes: In mouse spleen, a 30 kDa band is observed.
• Control:

  Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites with PBS
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-14 Antibody (70A1426) - BSA Free

• apoptosis-related cysteine protease
• CASP14
• CASP-14
• caspase 14, apoptosis-related cysteine peptidase
• caspase 14, apoptosis-related cysteine protease
• Caspase14
• Caspase-14
• EC 3.4.22.-
• MGC119078
• MGC119079
• MICE

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-14 is a newly identified member of Caspase family. It has conserved active site pentapeptide, QACRG. However, no proteolytic or biological activities have been identified so far. It's high expression in embryonic tissues and limited expression in adult tissues suggests that it may have some role during ontogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-14 Antibody (NB100-56718)(1)

Publication using NB100-56718

• Jung MH, Lee JH, Cho JG et al. Expressions of caspase-14 in human middle ear cholesteatoma. Laryngoscope. 2008-06-01 [PMID: 18520823] (IHC-P, WB)
  
  Details:
  Caspase-14 (IMG-167). WB: External auditory canal (EAC) epithelium and cholesteatoma tissue, Fig 2. IHC (paraffin): Normal EAC and cholesteatoma tissue Fig 3. Note: Caspase-14 (IMG-167) was used at a 1:500 dilution in WB, Fig 2.

Bioinformatics

• Gene Symbol: CASP14
• Entrez:
  • 12365(Mouse)
• Uniprot:
  • NP_033939(Mouse)