Caspase-11 Antibody

Western Blot: Caspase-11 Antibody [NBP1-45453] - Analysis of Caspase 11 with 3T3 cell lysate.

Product Discontinued

Caspase-11 Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab18741)
• Immunogen: Synthetic peptide made to the surrounding amino acid 257 of mouse or rat caspase-11. The sequence used as immunogen is identical among mouse and rat (NM_007609).
• Specificity: The antibody detects multiple products of Caspase 11 (also known as caspase 4 in mouse/rat): procaspase-11 (45 kDa), a partially cleaved fragment (30 kDa) and fully cleaved fragment (20 kDa). A 58 kDa unknown identity band was also detected in 3T3 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: Casp4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Western Blot 0.5 - 4 ug/mL
• Application Notes: Use in immunoblotting reported in scientific literature (PMID: 31698204).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5% BSA, 5 mM EDTA, 30% Glycerol
• Preservative: 0.03% Proclin 300
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Caspase-11 Antibody

• ich-3
• CASP11
• CASP-11
• CASP4
• CASP-4
• caspase 4, apoptosis-related cysteine peptidase
• Caspl

Background

This gene encodes a caspase that is involved in apoptosis, cell migration, and the inflammatory response. Although its official nomenclature is Casp4, caspase 4, apoptosis-related cysteine peptidase, this gene and its encoded protein have historically been called caspase 11. [provided by RefSeq, Feb 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Caspase-11 Antibody (NBP1-45453)(8)

Publications using NBP1-45453

• Alcarranza M, Villegas I, Recio R et al. (R)-8-Methylsulfinyloctyl isothiocyanate from Nasturtium officinale inhibits LPS-induced immunoinflammatory responses in mouse peritoneal macrophages: chemical synthesis and molecular signaling pathways involved Food & function 2023-07-31 [PMID: 37469300] (WB, Mouse)
• Muñoz-García R, Sánchez-Hidalgo M, Alcarranza M et al. Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice Antioxidants (Basel, Switzerland) 2023-06-19 [PMID: 37372034] (WB, Mouse)
• Xiao Q, Kang Z, Liu C et al. Panax notoginseng Saponins Attenuate Cerebral Ischemia-Reperfusion Injury via Mitophagy-Induced Inhibition of NLRP3 Inflammasome in Rats Frontiers in Bioscience-Landmark 2022-11-03 [PMID: 36472098] (WB, Rat)
  
  Details:
  Dilution used 1:500
• Tang B, She X, Deng C Effect of the combination of astragaloside IV and Panax notoginseng saponins on pyroptosis and necroptosis in rat models of cerebral ischemia reperfusion Experimental and Therapeutic Medicine 2021-08-04 [PMID: 34504577] (WB, Rat)
• Chang Y, Zhu J, Wang D et al. NLRP3 inflammasome-mediated microglial pyroptosis is critically involved in the development of post-cardiac arrest brain injury J Neuroinflammation 2020-07-23 [PMID: 32703306] (WB, Rat)
• Castejon ML, SAnchez-Hidalgo M, Aparicio-Soto M et al. Dietary oleuropein and its new acyl-derivate attenuate murine lupus nephritis through HO-1/Nrf2 activation and suppressing JAK/STAT, NF-kappa B, MAPK and NLRP3 inflammasome signaling pathways J. Nutr. Biochem. 2019-12-01 [PMID: 31698204] (IB, Mouse)
• Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke. J Biophotonics 2017-02-06 [PMID: 28164443] (WB, Mouse)
• Tomura S, de Rivero Vaccari JP, Keane RW et al. Effects of therapeutic hypothermia on inflammasome signaling after traumatic brain injury. J Cereb Blood Flow Metab. 2012-10-01 [PMID: 22781337] (WB, Rat)

Bioinformatics

• Gene Symbol: Casp4
• Entrez:
  • 12363(Mouse)
  • 114555(Rat)
• Uniprot:
  • NM_007609(Mouse)