Recombinant Human Caspase-10/FLICE2 Protein

Product Discontinued

Recombinant Human Caspase-10/FLICE2 Protein Summary

• Description: Recombinant protein for Human Caspase-10/FLICE2
  
  

  Source:E. coli.
  Amino Acid Sequence:(Q92851)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CASP10
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit of the recombinant caspase-10/b is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
  Molecular Weight: two large (18 ) and two small (11 ) subunits in a heterotetramer form.
  Specific activity: > 15000 units/mg
• Theoretical MW: 14.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized.
• Concentration: Lyoph
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1 unit per ul in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase-10/FLICE2 Protein

• Apoptotic protease Mch-4
• CASP10
• CASP-10
• caspase 10, apoptosis-related cysteine peptidase
• caspase 10, apoptosis-related cysteine protease
• Caspase10
• Caspase-10
• EC 3.4.22.63
• FADD-like ICE2
• FAS-associated death domain protein interleukin-1B-converting enzyme 2
• FLICE2
• ICE-like apoptotic protease 4
• interleukin-1B-converting enzyme 2
• Mch4
• MCH4ALPS2

Background

Caspase-10b (also known as FLICE 2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10/b was expressed in E. coli. The active caspase-10 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP10