Caspase-10/FLICE2 Activity Assay Kit (Colorimetric)

Caspase-10/FLICE2 Activity Assay Kit (Colorimetric) [NBP2-54829] - The assay was performed on murine insulinoma cell line following Novus' protocol.

Product Discontinued

Caspase-10/FLICE2 Activity Assay Kit (Colorimetric) Summary

• Description: Simple and convenient colorimetric assay to measure Caspase-10 activity in mammalian cells within 1-2 hrs. The assay kit provides a means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate AEVD-pNA.
  
  Detection method: Absorbance at 400 or 405 nmApplications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65663)
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP10

Applications/Dilutions

Reviewed Applications

Read 1 Review rated 5 using NBP2-54829 in the following application:

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-10/FLICE2 Activity Assay Kit (Colorimetric)

• Apoptotic protease Mch-4
• CASP10
• CASP-10
• caspase 10, apoptosis-related cysteine peptidase
• caspase 10, apoptosis-related cysteine protease
• Caspase10
• Caspase-10
• EC 3.4.22.63
• FADD-like ICE2
• FAS-associated death domain protein interleukin-1B-converting enzyme 2
• FLICE2
• ICE-like apoptotic protease 4
• interleukin-1B-converting enzyme 2
• Mch4
• MCH4ALPS2

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-10 is a 455 amino acid protein. However, caspase-10 produces, by alternative splicing, at least 12 different transcripts, all with introns, putatively encoding at least 11 different protein isoforms. For example, human caspase-10a is a 479 amino acid (aa) protein, and human caspase-10d is a 522 aa protein. The transcripts appear to differ by truncation of the end, presence or absence of 6 cassette exons, or common exons with different boundaries, because an internal intron is not always spliced out. Users are encoraged to go to the NCBI ACE View Data Base for more information. http://www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-10 gene mutations or polymorphisms have been associated with a number of diseases including type II autoimmune lymphoproliferative syndrome, non-Hiodgkin's lymphoma, and gastric cancer. It is thought that some mutations of the caspase-10 gene may lead to the loss of its apoptotic function, thereby contributing to the pathogenesis of some diseases. Caspase-10 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present. Recognizes caspase-10 forms that contain the peptide immunogen sequence (QPAFTLRKKLVFPVPLDALSI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Review for Caspase-10/FLICE2 Kit (NBP2-54829) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Francesca Pacifici 12/06/2018

Summary

• Lot: 3D25K01250

Comments

• Comments: The assay was performed on murine insulinoma cell line by following manufacturer's protocol.

Bioinformatics

• Gene Symbol: CASP10