Caspase-10/FLICE2 Assay Kit (Fluorometric)

Product Discontinued

Caspase-10/FLICE2 Assay Kit (Fluorometric) Summary

• Description: Simple and convenient fluorometric assay to measure Caspase-10 Activity within 1-2 hrs.
  
  Detection method: Fluorescence (Ex/Em 400/505 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65662)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP10

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-10/FLICE2 Assay Kit (Fluorometric)

• Apoptotic protease Mch-4
• CASP10
• CASP-10
• caspase 10, apoptosis-related cysteine peptidase
• caspase 10, apoptosis-related cysteine protease
• Caspase10
• Caspase-10
• EC 3.4.22.63
• FADD-like ICE2
• FAS-associated death domain protein interleukin-1B-converting enzyme 2
• FLICE2
• ICE-like apoptotic protease 4
• interleukin-1B-converting enzyme 2
• Mch4
• MCH4ALPS2

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). AEVD-AFC emits blue light (WLmax = 400 nm); upon cleavage of the substrate, free AFC emits a yellow-green fluorescence (WLmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microplate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP10