Caspase-10/FLICE2 Antibody

Western Blot: Caspase 10 Antibody [NB500-233] - WB detection of Caspase-10 in Jurkat cells using NB500-233 at a dilution of 1:500. Before making the lysates, the cells were treated with 2 uM staurosporine for different time periods as shown in the image.

Product Discontinued

Caspase-10/FLICE2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to N-terminus of Human Caspase 10
• Specificity: Reacts with the N-terminus of all isoforms of human Caspase-10.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: Western blot- Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with chicken, human, mouse, and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Caspase-10/FLICE2 Antibody

• Apoptotic protease Mch-4
• CASP10
• CASP-10
• caspase 10, apoptosis-related cysteine peptidase
• caspase 10, apoptosis-related cysteine protease
• Caspase10
• Caspase-10
• EC 3.4.22.63
• FADD-like ICE2
• FAS-associated death domain protein interleukin-1B-converting enzyme 2
• FLICE2
• ICE-like apoptotic protease 4
• interleukin-1B-converting enzyme 2
• Mch4
• MCH4ALPS2

Background

Caspases are death effector domain-containing cysteine aspartases presumed to be at or near the apex of apoptotic signaling pathways. All known isoforms of caspase-10 are abundantly expressed in fetal lung, kidney, and skeletal muscle but are very poorly expressed or absent in these tissues in the adult, implying a possible role for the caspase-10 family in fetal development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-10/FLICE2 Antibody (NB500-233)(1)

Publication using NB500-233

• Park SJ, Park SH, Kim JO et al. Carnitine sensitizes TRAIL-resistant cancer cells to TRAIL-induced apoptotic cell death through the up-regulation of Bax. Biochem Biophys Res Commun. 2012-11-09 [PMID: 23068102]

Bioinformatics

• Gene Symbol: CASP10
• Entrez:
  • 843(Human)