Caspase-10/FLICE2 Antibody (63131) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 63131
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Caspase-10/FLICE2 Antibody (63131) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Caspase-10/a/B (Mch 4)
  Val220-Ile479
  Accession # AAC50644
• Specificity: Detects human Caspase-10 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-10/FLICE2 Antibody (63131) [Alexa Fluor® 647]

• Apoptotic protease Mch-4
• CASP10
• CASP-10
• caspase 10, apoptosis-related cysteine peptidase
• caspase 10, apoptosis-related cysteine protease
• Caspase10
• Caspase-10
• EC 3.4.22.63
• FADD-like ICE2
• FAS-associated death domain protein interleukin-1B-converting enzyme 2
• FLICE2
• ICE-like apoptotic protease 4
• interleukin-1B-converting enzyme 2
• Mch4
• MCH4ALPS2

Background

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43  generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.