CASK Antibody (GT27)

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody [GT27] (NBP3-48791) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

CASK antibody [GT27] detects CASK protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CASK stained by CASK antibody [GT27] (NBP3-48791) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody [GT27] (NBP3-48791) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: GT27
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CASK Antibody (GT27) Summary

• Description: Novus Biologicals Mouse CASK Antibody (GT27) (NBP3-48791) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The immunogen used to generate this antibody corresponds to human CASK.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CASK Antibody (GT27)

• calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase
• Calcium/calmodulin-dependent serine protein kinase
• CAMGUK
• CMG
• EC 2.7.11
• EC 2.7.11.1
• FGS4CAGH39
• FLJ22219
• hCASK
• LIN2FLJ31914
• MICPCH
• peripheral plasma membrane protein CASK
• Protein lin-2 homolog
• TNRC8
• trinucleotide repeat containing 8

Background

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASK