Casein Kinase 2 beta Antibody

Western Blot: Casein Kinase 2 beta Antibody [NBP1-06515] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CKII beta antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Rb
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Casein Kinase 2 beta Antibody Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-1567, NB100-97835, NB200-171
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 165 and 215 of human casein kinase 2, beta using the numbering given in entry NP_001311.3 (GeneID 1460).
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSNK2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
• Control:

  Casein Kinase 2 beta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-1567, NB100-97835, NB200-171

Alternate Names for Casein Kinase 2 beta Antibody

• Casein Kinase 2 beta
• casein kinase 2, beta polypeptide
• Casein kinase II beta subunit
• casein kinase II subunit beta
• CK II beta
• CK2B
• CK2N
• CSK2B
• CSNK2B
• G5A
• MGC138222
• MGC138224
• Phosvitin
• Protein G5a

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 2 beta Antibody (NBP1-06515)(1)

Publication using NBP1-06515

• Golkowski M, Lius A, Sapre T et al. Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity Molecular Cell 2023-02-01 [PMID: 36736316] (IP, Human)

Bioinformatics

• Gene Symbol: CSNK2B
• Entrez:
  • 1460(Human)
  • 13001(Mouse)
• Uniprot:
  • NP_001311.3(Human)