Casein Kinase 2 alpha Antibody (844720) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF, KO
• Clone: 844720
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Casein Kinase 2 alpha Antibody (844720) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Casein Kinase 2α
  Asp253-Gln391
  Accession # P68400
• Specificity: Detects human Casein Kinase 2 alpha in ELISAs and Western blots. Detects mouse and rat Casein Kinase 2 alpha in Western blots
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSNK2A1
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Casein Kinase 2 alpha Antibody (844720) [Alexa Fluor® 350]

• Casein Kinase 2 alpha
• casein kinase 2, alpha 1 polypeptide
• casein kinase II alpha 1 subunit
• CK II alpha
• CK2 catalytic subunit alpha
• CK2A1
• CK2A1casein kinase II subunit alpha
• CKII
• CSNK2A1
• EC 2.7.11
• EC 2.7.11.1
• protein kinase CK2

Background

Casein kinase 2 (CK2) is a ubiquitous and constitutively active tetrameric serine/threonine kinase that is comprised of two catalytic subunits (CK2 alpha and/or CK2 alpha ‘) and two identical regulatory subunits (CK2 beta ). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role. The human CK2 alpha and CK2 alpha ‘ subunits are the products of two different genes. They have highly conserved catyalytic domains but divergent C-terminal regions. Within aa 253-391 (including the region of divergence between CK2 alpha and CK2 alpha ’), the 35-45 kDa human CK2 alpha shares 96% aa sequence identity with mouse and rat CK2 alpha . An alternatively spliced isoform of human CK2 alpha lacks the N-terminal 136 amino acids including a portion of the kinase domain. CK2 beta plays dual roles in the regulation of CK2 acivity. Its C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK2A1