Casein Kinase 1 alpha Antibody

Images

 
Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030] - Analysis of whole rat spinal cord homogenate stained with NBP2-50030, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular ...read more
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody [NBP2-50030] - HeLa cells stained with NBP2-50030 (red) and panspecific mouse monoclonal antibody to nuclear pore complexes NBP1-05390 (green). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, EqSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

Casein Kinase 1 alpha Antibody Summary

Immunogen
Recombinant full length Casein Kinase 1 alpha (shortest isoform). [UniProt# P48729]
Specificity
This antibody will bind to bind to all Casein Kinase 1 alpha isoforms.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
CSNK1A1
Purity
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:5000
  • Western Blot 1:10000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-50030.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Casein Kinase 1 alpha Antibody

  • Casein Kinase 1 alpha
  • casein kinase 1, alpha 1
  • casein kinase I isoform alpha
  • CK1CKI-alpha
  • CSNK1A1
  • down-regulated in lung cancer
  • EC 2.7.11.1
  • HLCDGP1
  • PRO2975

Background

Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Ialpha, Igamma, Idelta and Iepisilon, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 1 alpha Antibody (NBP2-50030)(1)

Reviews for Casein Kinase 1 alpha Antibody (NBP2-50030) (0)

There are no reviews for Casein Kinase 1 alpha Antibody (NBP2-50030). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Casein Kinase 1 alpha Antibody (NBP2-50030). (Showing 1 - 1 of 1 FAQ).

  1. Regarding Casein Kinase 1 alpha Antibody NBP2-50030: 1. The datasheet says the immunogen is recombinant full length Casein Kinase 1 alpha (shortest isoform) [UniProt# P48729], but why does the specificity say this antibody will bind to all Casein Kinase 1 isoforms. Is this a typo? I need the antibody specific to Casein Kinase 1 alpha but not Casein Kinase 1.2. The IHC application suggests a 1:5000 dilution, could I dilute 1:50 for storage? If yes, how long could it be stored?
    • The CK1a gene has two sites of alternate splicing which may or may not insert two sequences called L (long, 28 amino acids) and S (short, 12 amino acids). So there are four possible and actual expressed forms, CK1a, CK1aL, CK1aS and CK1aLS. With collaboration, the lab showed that the L peptide contained a nuclear localization sequence and, using GFP fusions, CK1a forms with L go into the nucleus and those without do not. These are the isoforms mentioned. However, cross reactivity with other isoforms of Casein Kinase 1 such as beta, gamma, delta, or epsilon has not been determined, and so we cannot say for sure whether cross reactivity will occur.

Secondary Antibodies

 

Isotype Controls

Additional Casein Kinase 1 alpha Products

Research Areas for Casein Kinase 1 alpha Antibody (NBP2-50030)

Find related products by research area.

Blogs on Casein Kinase 1 alpha

There are no specific blogs for Casein Kinase 1 alpha, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Casein Kinase 1 alpha Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CSNK1A1
Uniprot