Cardiac Troponin T Antibody (990033) [Unconjugated]

HAF018). A specific band was detected for Troponin T at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF018). A specific band was detected for Troponin T at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Troponin T at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

MAB18741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." > MAB18741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." title="Recombinant Human Troponin T protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse/Rat Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB18742) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." />

Recombinant Human Troponin T protein was serially diluted 2-fold and captured by Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # <a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 990033
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cardiac Troponin T Antibody (990033) [Unconjugated] Summary

• Immunogen: Human Troponin T (Cardiac) antigen
  Accession # P45379
• Specificity: Detects human Troponin T in ELISA. Detects human, mouse, and rat Troponin T in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 0.5-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cardiac Troponin T Antibody (990033) [Unconjugated]

• Cardiac Muscle Troponin T
• Cardiomyopathy, Dilated 1D (Autosomal Dominant)
• Cardiomyopathy, Hypertrophic
• CMD1D
• CMH2
• CMPD2
• cTnT
• LVNC6
• RCM3
• TNNT2
• TnTC
• troponin T type 2 (cardiac)
• Troponin T
• troponin T, cardiac muscle
• Troponin T2, Cardiac Type
• Truncated Cardiac Troponin T

Background

Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cardiac Troponin T Antibody (MAB18742)(2)

Publications using MAB18742

• Hamamura, K;Yoshida, Y;Oyama, K;Li, J;Kawano, S;Inoue, K;Toyooka, K;Yamadera, M;Matsunaga, N;Matsumura, T;Aritake, K; Hematopoietic Prostaglandin D Synthase Is Increased in Mast Cells and Pericytes in Autopsy Myocardial Specimens from Patients with Duchenne Muscular Dystrophy International journal of molecular sciences 2024-02-03 [PMID: 38339125] (Immunohistochemistry, Human)
• Gao R, Yang K, Qu Y et al. m6A demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3 iScience 2023-03-01 [PMID: 36876119]

Bioinformatics

• Gene Symbol: TNNT2
• Uniprot:
  • P45379()