CARD12 Antibody


Western Blot: CARD12 Antibody [NB100-56143] - NB100-56143 specifically recognizes NLRCR4, but not closely related NLR family members NOD1 and NOD2. HEK293T cells transiently transfected with human NOD1, NOD or NLRC4 more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

CARD12 Antibody Summary

A synthetic peptide corresponding to amino acids 971-989 (DFSTKEFLPDPALVRKLSQ) of human Ipaf/Clan/CARD12 was used as immunogen; GenBank no. gi|40788015|ref|NP_067032.3|.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200

Reactivity Notes

Cross reacts with Human.

Alternate Names for CARD12 Antibody

  • CARD12leucine rich repeat and CARD domaincontaining 4
  • caspase recruitment domain family, member 12
  • Caspase recruitment domain-containing protein 12
  • Clan protein
  • CLAN1ipaf
  • CLANCARD, LRR, and NACHT-containing protein
  • CLR2.1
  • Ice protease-activating factor
  • Ipaf
  • NLR family, CARD domain containing 4


Ipaf (also known as Clan/CARD12) is a CARD domain containing protein. CARD (caspase-associated recruitment domain) proteins are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). In general CARD proteins are implicated in host defense against infection, environmental stress or cellular damage. CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including Ipaf/Clan/CARD12. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. There are at least three major signaling pathways in which CARD proteins act: (1) Regulation of caspase activation in the context of apoptosis (2) Regulation of caspase activation in the context of inflammation (3) Regaultion of NF-kB activation in the context of innate or adaptive immune responses. As there is significant crosstalk between pathways that lead to caspase-mediated apoptosis or inflammation and pathways that result in NF-kB activation, it is logical that similar protein modules such as CARD domains are found repeatedly in proteins from all three pathways. Ipaf plays a role in regulating caspase-1 activity, which in turn mediates the maturation of inflammatory cytokines IL-1b and IL-18 (reviewed in Lu et al, 2005). In transfected cells, Ipaf has been shown to directly interact with procaspase-1 and induce proteolytic activation of procaspase-1 in transfected cells. On the flip side, macrophages from IPAF deficient mice failed to activate caspase-1 in response to Salmonella typhimurium infection underscoring the importance of IPAF in vivo. IPAF also interact with the pro-apoptotic adaptor protein ASC and co-expression of IPAF with ASC has been shown to induce NF-kB activation and apoptosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB
Species: Hu, Mu, Ca
Applications: WB, Flow, IHC, IHC-Fr, IHC-P, Flow-CS, Flow-IC
Species: Hu
Applications: WB, IHC-P
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Mu
Applications: WB, IHC
Supplier Logo

Publications for CARD12 Antibody (NB100-56143) (0)

There are no publications for CARD12 Antibody (NB100-56143).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CARD12 Antibody (NB100-56143) (0)

There are no reviews for CARD12 Antibody (NB100-56143). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CARD12 Antibody (NB100-56143). (Showing 1 - 1 of 1 FAQ).

  1. I have purchased one of your antibodies (NB100-56143, polyclonal antibody to NLRC4) and require additional information, I have performed a western blot and detected multiple bands, I am unsure if these bands are breakdown products or detection of splice variants of the protein. In short, does your antibody detect NLRC4 splice variants?
    • This antibody was made against the following peptide immunogen sequence: DFSTKEFLPDPALVRKLSQ. The antibody would be expected to recognize any NLRC4 forms that contain the sequence used for immunogen. I did a quick blast search ( and 345, 359, 404 and 989 amino acid forms containing this the immunogen sequence were identified. Of course, all proteins can have post-translational modifications and cleavage forms which can contribute to variable banding patterns. The IMG-5730 antibody has been evaluated with over expressed NLRC4 as shown on the data data sheet, but not with endogenous lysate, so I am not sure what the of endogenous NLRC4 is expressed or what size endogenous NLRC4 bands would typically be recognized with this antibody in a given lysate.

Secondary Antibodies


Isotype Controls

Additional CARD12 Antibody Products

Related Products by Gene

Bioinformatics Tool for CARD12 Antibody (NB100-56143)

Discover related pathways, diseases and genes to CARD12 Antibody (NB100-56143). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CARD12 Antibody (NB100-56143)

Discover more about diseases related to CARD12 Antibody (NB100-56143).

Pathways for CARD12 Antibody (NB100-56143)

View related products by pathway.

PTMs for CARD12 Antibody (NB100-56143)

Learn more about PTMs related to CARD12 Antibody (NB100-56143).

Research Areas for CARD12 Antibody (NB100-56143)

Find related products by research area.

Blogs on CARD12

There are no specific blogs for CARD12, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol NLRC4

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-56143 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought