CARD11/CARMA1 Antibody

Images

 
Western Blot: CARD11/CARMA1 Antibody [NB100-1200] - Staining of Human Jurkat cells (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [NB100-1200] - Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CARD11/CARMA1 Antibody Summary

Immunogen
Peptide with sequence C-QRKTIWVDEDQL corresponding to C-Terminus according to NP_115791.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CARD11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. IHC: Paraffin embedded human spleen shows nuclear staining.
Control
CARD11/CARMA1 Lysate (NBL1-08695)
Publications
Read Publications using
NB100-1200 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine. Mouse reactivity reported in scientific literature (PMID: 24036998).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CARD11/CARMA1 Antibody

  • BIMP3
  • BIMP3card-maguk protein 1
  • CARD11
  • CARD-containing MAGUK protein 1
  • Carma 1
  • CARMA1
  • CARMA1bcl10-interacting maguk protein 3
  • caspase recruitment domain family, member 11
  • caspase recruitment domain-containing protein 11
  • MGC133069

Background

This protein belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CARD11/CARMA1 Antibody (NB100-1200)(2)

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CARD11
Entrez
Uniprot