Carboxypeptidase M Antibody (848543) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 848543
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Carboxypeptidase M Antibody (848543) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Carboxypeptidase M
  Leu18-Ser423
  Accession # P14384
• Specificity: Detects human Carboxypeptidase M in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase M Antibody (848543) [Alexa Fluor® 488]

• Carboxypeptidase M
• CPM
• EC 3.4.17
• EC 3.4.17.12

Background

Carboxypeptidase M (CPM) is a 50‑65 kDa monomer that belongs to the regulatory CPN/E subfamily, peptidase M14 family of enzymes. It is widely expressed, being found on macrophages, fibroblasts, endothelial cells, oligodendrocytes and Schwann cells, dendritic cells, osteoblasts and bronchial epithelium. Carboxypeptidase M is a GPI‑linked glycoprotein that is best known as a peptidase that cleaves basic amino acids (aa) from the carboxyterminal of a number of peptides, including EGF and bradykinin. It is also known to bind to apparent substrates and undergo a conformational change that links it with the kinin B1 GPCR, initiating signal transduction. Mature human Carboxypeptidase M is 406 aa in length (aa 18-423). It contains one large enzymatic region (aa 19-310) and two critical glutamic acid residues at Glu260 and Glu264. Like many GPI‑linked proteins, Carboxypeptidase M undergoes solubilization and is reportedly found in urine and amniotic fluid. Over aa 18-423 (mature Carboxypeptidase M), human Carboxypeptidase M shares 86% aa sequence identity with mouse Carboxypeptidase M.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.