Carboxypeptidase M Antibody (848543)

Western blot shows lysates of human placenta tissue and human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Carboxypeptidase M Monoclonal Antibody (Catalog # MAB7457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carboxypeptidase M at approximately 58-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Carboxypeptidase M was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Carboxypeptidase M Monoclonal Antibody (Catalog # MAB7457) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 848543
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carboxypeptidase M Antibody (848543) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Carboxypeptidase M
  Leu18-Ser423
  Accession # P14384
• Specificity: Detects human Carboxypeptidase M in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CPM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase M Antibody (848543)

• Carboxypeptidase M
• CPM
• EC 3.4.17
• EC 3.4.17.12

Background

Carboxypeptidase M (CPM) is a 50‑65 kDa monomer that belongs to the regulatory CPN/E subfamily, peptidase M14 family of enzymes. It is widely expressed, being found on macrophages, fibroblasts, endothelial cells, oligodendrocytes and Schwann cells, dendritic cells, osteoblasts and bronchial epithelium. Carboxypeptidase M is a GPI‑linked glycoprotein that is best known as a peptidase that cleaves basic amino acids (aa) from the carboxyterminal of a number of peptides, including EGF and bradykinin. It is also known to bind to apparent substrates and undergo a conformational change that links it with the kinin B1 GPCR, initiating signal transduction. Mature human Carboxypeptidase M is 406 aa in length (aa 18-423). It contains one large enzymatic region (aa 19-310) and two critical glutamic acid residues at Glu260 and Glu264. Like many GPI‑linked proteins, Carboxypeptidase M undergoes solubilization and is reportedly found in urine and amniotic fluid. Over aa 18-423 (mature Carboxypeptidase M), human Carboxypeptidase M shares 86% aa sequence identity with mouse Carboxypeptidase M.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPM
• Uniprot:
  • P14384(Human)