Carboxypeptidase B1/CPB1 Overexpression Lysate


Western Blot: Carboxypeptidase B Overexpression Lysate (Adult Normal) [NBL1-09429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carboxypeptidase B.

Product Details

Reactivity HuSpecies Glossary
Applications WB

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Carboxypeptidase B1/CPB1 Overexpression Lysate Summary


Carboxypeptidase B1/CPB1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC204463

Accession#: NM_001871

Protein Tag: C-MYC/DDK


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
45.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Carboxypeptidase B1/CPB1 Overexpression Lysate

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B


Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Carboxypeptidase B1/CPB1 Lysate (NBL1-09429) (0)

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FAQs for Carboxypeptidase B1/CPB1 Lysate (NBL1-09429). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit .
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

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Gene Symbol CPB1