Carboxypeptidase A1/CPA1 Antibody (OTI2A3)

Western Blot: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Carboxypeptidase A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Carboxypeptidase A.

Immunocytochemistry/Immunofluorescence: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining (Red) of COS7 cells transiently transfected by pCMV6-ENTRY Carboxypeptidase A.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded liver using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Adenocarcinoma of breast using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Adenocarcinoma of ovary using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded breast using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Carcinoma of kidney using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Carcinoma of liver using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded Carcinoma of pancreas using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded endometrium using anti-Carboxypeptidase A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Carboxypeptidase A1/CPA1 Antibody (2A3) [NBP1-47704] - Staining of paraffin-embedded kidney using anti-Carboxypeptidase A mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI2A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Carboxypeptidase A1/CPA1 Antibody (OTI2A3) Summary

• Description: Novus Biologicals Mouse Carboxypeptidase A1/CPA1 Antibody (OTI2A3) (NBP1-47704) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Carboxypeptidase A1/CPA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-419 of human CPA-1
• Specificity: This antibody is specific for Homo sapiens carboxypeptidase A1 (pancreatic) (CPA1).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 45.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Carboxypeptidase A1/CPA1 Antibody (OTI2A3)

• carboxypeptidase A1 (pancreatic)
• Carboxypeptidase A1
• CPA
• CPA1
• EC 3.4.17
• EC 3.4.17.1

Background

Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties. Carboxypeptidase A1 is a monomeric pancreatic exopeptidase. It is involved in zymogen inhibition

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carboxypeptidase A1/CPA1 Antibody (NBP1-47704)(1)

Publication using NBP1-47704

• Villani V, Thornton ME, Zook HN et al. SOX9+/PTF1A+ Cells Define the Tip Progenitor Cells of the Human Fetal Pancreas of the Second Trimester Stem Cells Transl Med 2019-10-21 [PMID: 31631582] (IF/IHC, Human)

Review for Carboxypeptidase A1/CPA1 Antibody (NBP1-47704) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 11/16/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human pancreas
• Species: Human
• Lot: A001

FAQs for Carboxypeptidase A1/CPA1 Antibody (NBP1-47704). (Showing 1 - 1 of 1 FAQ).

1. I would like to know if anybody has tried to use this anti-Carboxypeptidase A antibody (NBP1-47704) for immunohistochemistry in mouse tissues?
   • This antibody has never been tested by our lab or reported to us by a customer for staining of mouse tissues. The most likely reason for this isn't necessarily the sequence homology, but due to the fact that this is a mouse monoclonal antibody staining on tissues of the same host can be problematic because non-specific binding of the secondary could cause high background and make interpretation of your samples difficult.

Bioinformatics

• Gene Symbol: CPA1
• Entrez:
  • 1357(Human)
• Uniprot:
  • P15085()