Carbonic Anhydrase VII/CA7 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Carbonic Anhydrase VII/CA7 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CA7.
  
  

  Source: E. coli

  Amino Acid Sequence: PINIISSQAVYSPSLQPLELSYEACMSLSITNNGHSVQVDFNDSDDRTVV

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CA7
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-30702.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Carbonic Anhydrase VII/CA7 Recombinant Protein Antigen

• CA7
• Carbonate dehydratase VII
• carbonic anhydrase 7
• Carbonic Anhydrase VII
• carbonic anhydrase VIICAVII
• carbonic dehydratase VII
• CA-VII
• EC 4.2.1.1

Background

CA7, also known as Carbonic Anhydrase 7, consists of a 29 kDa and a shorter 23 kDa isoform, is involved in zinc ion binding as a member of the zinc metalloenzyme family that transports HCO3- and a proton (H+) into H20 and CO2. This process occurs in several pathways such as the metabolism and reversible hydration of carbon dioxide pathways. Disease research is currently being studied with relation to CA7 and colorectal cancer, glaucoma, insulin resistance and neuronitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CA7