| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | DyLight 488 |
| Immunogen | Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VA/CA5A (NP_001730.1; Ala 40-Ser 305). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CA5A |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Affinity purified |
![Carbonic Anhydrase VA/CA5A Antibody [DyLight 488]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Carbonic Anhydrase VA/CA5A Antibody [DyLight 488]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-99341AF350 | |
| Alexa Fluor 405 | NBP2-99341AF405 | |
| Alexa Fluor 488 | NBP2-99341AF488 | |
| Alexa Fluor 532 | NBP2-99341AF532 | |
| Alexa Fluor 594 | NBP2-99341AF594 | |
| Alexa Fluor 647 | NBP2-99341AF647 | |
| Alexa Fluor 700 | NBP2-99341AF700 | |
| Alexa Fluor 750 | NBP2-99341AF750 | |
| Biotin | NBP2-99341B | |
| DyLight 350 | NBP2-99341UV | |
| DyLight 405 | NBP2-99341V | |
| DyLight 488 | NBP2-99341G | |
| DyLight 550 | NBP2-99341R | |
| DyLight 594 | NBP2-99341DL594 | |
| DyLight 650 | NBP2-99341C | |
| DyLight 680 | NBP2-99341FR | |
| DyLight 755 | NBP2-99341IR | |
| FITC | NBP2-99341F | |
| HRP | NBP2-99341H |
Research Areas for Carbonic Anhydrase VA/CA5A Antibody (NBP2-99341G)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CA5A |
![Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000. Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.](http://images.novusbio.com/images2/Caveolin-1-Antibody-(7C8)-Western-Blot-NB100-615-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caveolin-1 Antibody (7C8)-Immunocytochemistry-Immunofluorescence-NB100-615-img0014.jpg)
![Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/Caveolin-1-Antibody-(7C8)-Immunohistochemistry-Frozen-NB100-615-img0015.jpg)
![Western Blot: Caveolin-3 Antibody [NB110-5029] - Analysis of rat cardiac muscle protein extract.](http://images.novusbio.com/images2/Caveolin-3-Antibody-Western-Blot-NB110-5029-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-3 Antibody [NB110-5029] - C2C11 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Caveolin 3 polyclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/images2/Caveolin-3-Antibody-Immunocytochemistry-Immunofluorescence-NB110-5029-img0010.jpg)
![Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse skeletal muscle tissue.](http://images.novusbio.com/images2/Caveolin-3-Antibody-Immunohistochemistry-Paraffin-NB110-5029-img0013.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Expression of endothelial markers indicate functional endothelium in TEVG. Whole mount staining of native aorta and TEVG. VE-cadherin is a marker of cellular borders of endothelial cells (green). Endothelial nitric oxide synthase (eNOS) is a marker of a functional endothelium (red). DAPI is a nuclear stain (blue). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120328), licensed under a CC-BY licence.](http://images.novusbio.com/images2/eNOS Antibody-Immunohistochemistry-Paraffin-NB300-500-img0006.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.](http://images.novusbio.com/images2/eNOS-Antibody-Immunohistochemistry-Paraffin-NB300-500-img0003.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.](http://images.novusbio.com/images2/eNOS-Antibody-Immunohistochemistry-Paraffin-NB300-500-img0004.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.](http://images.novusbio.com/images2/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Caveolin-2 Antibody [NBP1-31116] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caveolin 2 protein stained by Caveolin 2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Caveolin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31116-img0009.jpg)
![Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116] - Paraffin-embedded FaDu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Caveolin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31116-img0006.jpg)
![Western Blot: Nanos3 Antibody [NBP1-76919] - Human brain tissue lysate with Nanos3 antibody at 2 ug/mL.](http://images.novusbio.com/images2/Nanos3-Antibody-Western-Blot-NBP1-76919-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nanos3 Antibody [NBP1-76919] - in Human Brain cells.](http://images.novusbio.com/images2/Nanos3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76919-img0003.jpg)
![Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP1-76919] - Human brain tissue with Nanos3 antibody at 2.5 ug/ml.](http://images.novusbio.com/images2/Nanos3-Antibody-Immunohistochemistry-Paraffin-NBP1-76919-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AP3B1 Antibody [NBP1-89228] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/images2/AP3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89228-img0002.jpg)
![Immunohistochemistry-Paraffin: AP3B1 Antibody [NBP1-89228] - Staining of human rectum shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/AP3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89228-img0003.jpg)
![Western Blot: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/images2/Prolyl-Oligopeptidase-PREP-Antibody-Western-Blot-NBP2-33950-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody stainin is shown in green.](http://images.novusbio.com/images2/Prolyl-Oligopeptidase-PREP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33950-img0005.jpg)
![Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Staining in human colon and pancreas tissues using anti-PREP antibody. Corresponding PREP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Prolyl-Oligopeptidase-PREP-Antibody-Immunohistochemistry-Paraffin-NBP2-33950-img0008.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![ELISA: FMN1 Antibody [44110002]](http://images.novusbio.com/images/44110002-T01565A01.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Electrophysiological properties of colonic myenteric neurons from non-treated & chemotherapy-treated patients. Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired a single action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A](http://images.novusbio.com/images2/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0005.jpg)
![Immunohistochemistry: nNOS Antibody [NB100-858] - Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse caudate-putamen. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Scale bar: 50 um; inset: 20 um. Data obtained by Drs. Eva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.](http://images.novusbio.com/images2/nNOS-Antibody-Immunohistochemistry-NB100-858-img0001.jpg)
![Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/nNOS-Antibody-Flow-Cytometry-NB100-858-img0003.jpg)
![Western Blot: FoxC2 Antibody [NB100-1269] - Staining of nuclear HEK293 cell lysate with antibody at 2 ug/mL (A) and negative control human pancreas cell lysate with antibody at 1 ug/mL (B) (RIPA buffer 35 ug total protein per lane). Detected by chemiluminescence.](http://images.novusbio.com/images2/FoxC2-Antibody-Western-Blot-NB100-1269-img0012.jpg)
![Flow Cytometry: FoxC2 Antibody [NB100-1269] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/FoxC2-Antibody-Flow-Cytometry-NB100-1269-img0011.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/images2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0010.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0016.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Western-Blot-NBP1-47842-img0011.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Immunohistochemistry-Paraffin-NBP1-47842-img0019.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Western-Blot-NBP1-47842-img0010.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0017.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0018.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005555-P01-1.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/images2/AKT1-[p-Ser473]-Antibody-(104A282)-Western-Blot-NB100-56749-img0014.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/images2/AKT1-[p-Ser473]-Antibody-(104A282)-Western-Blot-NB100-56749-img0015.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/images2/AKT1-[p-Ser473]-Antibody-(104A282)-Immunohistochemistry-Paraffin-NB100-56749-img0009.jpg)