Recombinant Human CaM Kinase I Protein

Product Discontinued

Recombinant Human CaM Kinase I Protein Summary

• Description: Recombinant protein for Human CaM Kinase I
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(Q14012)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60898)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: CAMK1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 138 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human CaM Kinase I Protein

• calcium/calmodulin-dependent protein kinase I
• calcium/calmodulin-dependent protein kinase type 1
• CaM kinase I alpha
• CaM Kinase I
• CAMKI
• caM-KI
• caMKI-alpha
• EC 2.7.11
• EC 2.7.11.17
• MGC120317
• MGC120318

Background

Calcium/calmodulin-dependent protein kinase ID (CAMK1D), or a novel Ca2+/calmodulin-dependent kinase I-like kinase (CKLiK), showed kinase activity and that the activity was enhanced by Ca(2+) and calmodulin. Using a novel antibody generated against the C-terminus of CKLiK, CKLiK was detected in CD34+-derived neutrophils and eosinophils, as well as in mature peripheral blood granulocytes. Activation of human granulocytes by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF), but not the phorbol ester PMA (phorbol 12-myristate-13-acetate), resulted in induction of CKLiK activity, in parallel with a rise of intracellular [Ca2+]. Furthermore, fMLP-induced neutrophil migration on albumin-coated surfaces was perturbed, as well as Beta 2-integrin-mediated adhesion. These findings suggest a critical role for CKLiK in modulating chemoattractant-induced functional responses in human granulocytes (1). Also, CAMK1D exhibits Ca(2+)/CaM-dependent activity that is enhanced (approximately 30-fold) in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)a, consistent with detection of CAMK1D-activating activity in HeLa cells. Transiently expressed CAMK1D exhibited enhanced protein kinase activity in HeLa cells without ionomycin stimulation. This sustained activation of CAMK1D was completely abolished by Thr180Ala mutation and inhibited by CaM-KK inhibitor, STO-609, indicating a functional CaM-KK/ CAMK1D cascade in HeLa cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK1