Recombinant Human CaM Kinase I GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, MA, AP, Enzyme Activity

Recombinant Human CaM Kinase I GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-370 of Human CAMK1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASHGELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQL

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: CAMK1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence
  • Kinase Assay
  • Protein Array
  • Western Blot
• Theoretical MW: 66.33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CaM Kinase I GST (N-Term) Protein

• calcium/calmodulin-dependent protein kinase I
• calcium/calmodulin-dependent protein kinase type 1
• CaM kinase I alpha
• CaM Kinase I
• CAMKI
• CaM-KI
• caMKI-alpha
• EC 2.7.11
• EC 2.7.11.17
• MGC120317
• MGC120318

Background

CAMK1( NP_003647, 1 a.a. - 371 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CaMKI Recombinant Protein (H00008536-P01)(2)

Publications using H00008536-P01

• Watanabe S, Horie T, Nagao K et al. Cardiac-Specific Inhibition of Kinase Activity in Calcium/Calmodulin-Dependent Protein Kinase Kinase-b Leads to Accelerated Left Ventricular Remodeling and Heart Failure after Transverse Aortic Constriction in Mice. PLoS One. 2014-09-25 [PMID: 25255457]
• Mallampalli RK, Kaercher L, Snavely C et al. Fbxl12 triggers G1 arrest by mediating degradation of calmodulin kinase I. Cell Signal. 2013-05-23 [PMID: 23707388]

Bioinformatics

• Gene Symbol: CAMK1