Calsequestrin 1 Antibody (JB57-37)

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody (JB57-37) [NBP2-75429] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Calsequestrin 1 Antibody (JB57-37) [NBP2-75429] - Analysis of C2C12 cells with Calsequestrin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody (JB57-37) [NBP2-75429] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody (JB57-37) [NBP2-75429] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Calsequestrin 1 antibody. Counter stained with hematoxylin.

Product Discontinued

Calsequestrin 1 Antibody (JB57-37) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Calsequestrin 1 aa 80-220 / 396. (SwissProt: P31415 Human; SwissProt: O09165 Mouse; SwissProt: P19633 Rat)
• Localization: Endoplasmic reticulum. Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CASQ1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calsequestrin 1 Antibody (JB57-37)

• calmitin
• calmitine
• calsequestrin 1 (fast-twitch, skeletal muscle)
• Calsequestrin, skeletal muscle isoform
• calsequestrin-1
• CASQ
• PDIB1

Background

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASQ1