Calpain 2 Antibody

Western Blot: Calpain 2 Antibody [NBP2-15675] - Sample (30 ug of whole cell lysate) A:NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP2-15675] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP2-15675] - Paraffin-embedded mouse prostate. Calpain 2 antibody [N2C1], Internal dilution: 1:500.

Western Blot: Calpain 2 Antibody [NBP2-15675] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Discontinued

Calpain 2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.
• Localization: Cytoplasm; Cell membrane
• Predicted Species: Porcine (92%), Bovine (91%), Rabbit (92%), Rhesus Macaque (98%), Sheep (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (89%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Calpain 2 Antibody

• Calcium-activated neutral proteinase 2
• calpain 2, (m/II) large subunit
• calpain 2, large [catalytic] subunit
• calpain 2, large subunit
• Calpain large polypeptide L2
• Calpain M-type
• calpain, large polypeptide L2
• calpain-2 catalytic subunit
• Calpain-2 large subunit
• CANP 2
• CANPL2CANP2
• CANPml
• EC 3.4.22
• EC 3.4.22.53
• FLJ39928
• M-calpain
• mCANP
• millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Calpain 2 Antibody (NBP2-15675)(2)

Publications using NBP2-15675

• Li Y, Liu C, Li B et al. Electrical stimulation activates calpain 2 and subsequently upregulates collagens via the integrin b1/TGF-b1 signaling pathway Cell. Signal. 2019-03-30 [PMID: 30940604] (WB, Mouse)
• Zuo J, Hu Z, Liu T et al. Calpeptin attenuates cigarette smoke-induced pulmonary inflammation via suppressing calpain/IkBa signaling in mice and BEAS-2B cells. Pathology - Research and Practice 2018-08-01 [PMID: 30078403] (WB, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: CAPN2
• Uniprot:
  • P17655(Human)