Calpain 2 Antibody (JA43-41)

Western Blot: Calpain 2 Antibody (JA43-41) [NBP2-67202] - Analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody (JA43-41) [NBP2-67202] - Staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Calpain 2 Antibody (JA43-41) [NBP2-67202] - Analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Calpain 2 Antibody (JA43-41) [NBP2-67202] - Analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Calpain 2 Antibody (JA43-41) [NBP2-67202] - Analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.

Product Discontinued

Calpain 2 Antibody (JA43-41) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Calpain 2 aa 651-700 / 700. (SwissProt: P17655 Human; SwissProt: O08529 Mouse; SwissProt: Q07009 Rat)
• Localization: Cytoplasm. Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAPN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calpain 2 Antibody (JA43-41)

• Calcium-activated neutral proteinase 2
• calpain 2, (m/II) large subunit
• calpain 2, large [catalytic] subunit
• calpain 2, large subunit
• Calpain large polypeptide L2
• Calpain M-type
• calpain, large polypeptide L2
• calpain-2 catalytic subunit
• Calpain-2 large subunit
• CANP 2
• CANPL2CANP2
• CANPml
• EC 3.4.22
• EC 3.4.22.53
• FLJ39928
• M-calpain
• mCANP
• millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPN2