Calpain 1 Antibody (156)

Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (156) [NB600-1404] - Analysis of Calpain in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Calpain monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Product Discontinued

Calpain 1 Antibody (156) Summary

• Immunogen: Purified bovine skeletal muscle 28 kDa calpain subunit.
• Specificity: Detects Calpain 28 kDa subunit from bovine platelet, skeletal and heart muscle and human tissues. This does not cross-react with the human 80 kDa m-Calpain subunit.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAPN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Western Blot 1:1000
• Application Notes: WB: Detects a 28 kDa protein representing the 28 kDa subunit of calpain from bovine heart extract.
• Control:

  Calpain 1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Calpain 1 Antibody (156)

• Calcium-activated neutral proteinase 1
• Calpain 1
• calpain 1, (mu/I) large subunit
• Calpain mu-type
• calpain, large polypeptide L1
• calpain-1 catalytic subunit
• Calpain-1 large subunit
• CANP 1
• CANP
• CANPL1
• CANPL1EC 3.4.22.52
• CAPN1
• Cell proliferation-inducing gene 30 protein
• cell proliferation-inducing protein 30
• EC 3.4.22
• EC 3.4.22.52
• Micromolar-calpain
• muCANP
• muCANPCANP1
• muCL
• PIG30

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPN1
• Entrez:
  • 823(Human)