Human Caldesmon/CALD1 ELISA Kit (Colorimetric)

ELISA: Human Caldesmon/CALD1 ELISA Kit (Colorimetric) [NBP2-74969] - Samples were spiked with high concentrations of Human Caldesmon/CALD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Caldesmon/CALD1 ELISA Kit (Colorimetric) [NBP2-74969] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Caldesmon/CALD1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CALD in samples. No significant cross-reactivity or interference between Human CALD and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.29%
• Intra-Assay: CV% < 5.74%
• Spike Recovery: 86-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CALD1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Caldesmon/CALD1 ELISA Kit (Colorimetric)

• CAD
• CALD1
• caldesmon 1
• Caldesmon
• CDM
• CDMH-CAD
• HCAD
• LCAD
• L-CAD
• MGC21352
• NAG22

Background

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CALD1