Caldesmon/CALD1 Antibody (SU03-19)

Western Blot: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Analysis of Caldesmon on NIH/3T3 cell lysates using anti-Caldesmon antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.

Flow Cytometry: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SU03-19) [NBP2-67458] - Analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.

Product Discontinued

Caldesmon/CALD1 Antibody (SU03-19) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Caldesmon/CALD1 aa 744-793 / 793. (SwissProt: Q05682 Human; Unigene: 308134 Mouse; SwissProt: Q62736 Rat)
• Localization: Cytoplasm, Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CALD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Caldesmon/CALD1 Antibody (SU03-19)

• CAD
• CALD1
• caldesmon 1
• Caldesmon
• CDM
• CDMH-CAD
• HCAD
• LCAD
• L-CAD
• MGC21352
• NAG22

Background

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALD1