Caldesmon/CALD1 Antibody (767001) [Alexa Fluor® 594]

• Applications: WB, IHC, ICC/IF
• Clone: 767001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Caldesmon/CALD1 Antibody (767001) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Caldesmon/CALD1
  Lys696-Val793
  Accession # Q05682
• Specificity: Detects human Caldesmon/CALD1 in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Caldesmon, also known as CaD or h-CaD, is a 120-150 kDa cytosolic protein that is important in actin cytoskeleton dynamics. Smooth muscle Caldesmon regulates calcium-dependent smooth muscle contraction by stabilizing actin filaments, inhibiting the actomyosin ATPase, and interacting with F-actin, myosin, tropomyosin, and calmodulin. It is also a key molecule in other related actin-related processes including cytokinesis, cell migration, wound healing, and exocytosis. Caldesmon activity is regulated by serine and tyrosine phosphorylation by multiple kinases. Alternate splicing generates 70-80 kDa isoforms of human Caldesmon, known as I-CaD, that lack the central repeating region of the protein (aa 208-436 or aa 208-462). These isoforms are widely expressed and are comparable in size to full length mouse and rat Caldesmon. Within aa 696-793, human Caldesmon shares 94% and 95% aa sequence identity with mouse and rat Caldesmon, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.