Calcium Activated Nucleotidase 1/CANT1 Antibody (2D3) - Azide and BSA Free

Western Blot: Calcium Activated Nucleotidase 1/CANT1 Antibody (2D3) [H00124583-M01] - CANT1 monoclonal antibody (M01), clone 2D3 Analysis of CANT1 expression in A-431.

Western Blot: Calcium Activated Nucleotidase 1/CANT1 Antibody (2D3) [H00124583-M01] - Analysis of CANT1 expression in transfected 293T cell line by CANT1 monoclonal antibody (M01), clone 2D3.Lane 1: CANT1 transfected lysate(44.8 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Calcium Activated Nucleotidase 1/CANT1 Antibody (2D3) [H00124583-M01] - Detection limit for recombinant GST tagged CANT1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Calcium Activated Nucleotidase 1/CANT1 Antibody (2D3) - Azide and BSA Free Summary

• Immunogen: CANT1 (AAH17655, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI
• Specificity: CANT1 - calcium activated nucleotidase 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CANT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Calcium Activated Nucleotidase 1/CANT1 Antibody (2D3) - Azide and BSA Free

• Apyrase homolog
• calcium activated nucleotidase 1
• CANT1
• DBQD
• EC 3.6.1.6
• Putative MAPK-activating protein PM09
• Putative NF-kappa-B-activating protein 107
• SCAN1
• SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase
• SHAPY
• SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification
• soluble Ca-activated nucleotidase, isozyme 1
• soluble calcium-activated nucleotidase 1
• soluble calcium-activated nucleotidase SCAN-1

Background

Calcium-activated nucleotidase-1 (EC 3.6.1.6) is an extracellular protein that functions as a nucleotide tri- anddiphosphatase (Smith et al., 2002 (PubMed 12234496)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calcium Activated Nucleotidase 1/CANT1 Antibody (H00124583-M01)(2)

Publications using H00124583-M01

• Liu X, Yang Z, Luo X et al. Calcium-activated nucleotidase 1 silencing inhibits proliferation, migration, and invasion in human clear cell renal cell carcinoma. J Cell Physiol. 2019-05-17 [PMID: 31102300]
• Scheble VJ, Braun M, Wilbertz T et al. ERG rearrangement in small cell prostatic and lung cancer. Histopathology. 2010-01-10 [PMID: 20636794]

Bioinformatics

• Gene Symbol: CANT1
• Entrez:
  • 124583(Human)
• Uniprot:
  • AAH17655(Human)