Cadherin-6/KCAD Antibody (HKT288) - Low Endotoxin, Azide and BSA Free

Immobilized Cyno Cadherin-6/KCAD His at 2 ug/mL can bind Cadherin-6/KCAD Antibody (HKT288), EC50=0.006944 ug/mL.

The endocytosis ratio HKT288 by Hu-CDH6-HEK293(A6) increased with the increase of antibody concentration, and the Internalization Rate (%) reached 93% at antibody concentration of 2 nM.

HKT288 induced OVCAR3 Luciferase activity was evaluated using huCD16a(V158)-NF-AT-Jurkat Reporter Cell. The EC50 was approximately 0.6792 nM.

Human Cadherin-6/KCAD HEK293 (A6) cells were stained with Cadherin-6/KCAD Antibody (HKT288) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.08624 ug/mL.

Mouse CDH6 / K-Cadherin HEK293 Cell Line were stained with HKT288 and negative control protein respectively, washed and then followed by PE and analyzed with FACS,EC50=0.1068 ug/mL.

Cynomolgus / Rhesus Macaque CDH6 / K-Cadherin HEK293 Cell Line were stained with HKT288 and negative control protein respectively, washed and then followed by PE and analyzed with FACS,EC50=0.1818 ug/mL.

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clone: HKT288
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated
• Format: Low Endotoxin, Azide and BSA Free
• Concentration: LYOPH

Cadherin-6/KCAD Antibody (HKT288) - Low Endotoxin, Azide and BSA Free Summary

• Description: The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
  
  This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
  
  Patent #: WO2018185618
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: CDH6 / K-Cadherin
• Source: CHO
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: CDH6
• Purity: Protein A purified
• Endotoxin Note: < 0.001EU/ug,determined by LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional

Packaging, Storage & Formulations

• Storage: Store at -20C in powder form. Store at -80C once reconstituted.
• Buffer: Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Alternate Names for Cadherin-6/KCAD Antibody (HKT288) - Low Endotoxin, Azide and BSA Free

• cadherin 6, type 2, K-cadherin (fetal kidney)
• cadherin, fetal kidney
• Cadherin6
• Cadherin-6
• CDH6
• KCAD
• K-cadherin (fetal kidney)
• K-Cadherin

Background

K Cadherin encodes a type II classical cadherin from the cadherin superfamily. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures such as epithelium. Strong transcriptional expression of this gene has been observed in hepatocellular and renal carcinoma cell lines, suggesting a possible role in metastasis and invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH6
• Uniprot:
  • P55285()