Cadherin-4/R-Cadherin Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cadherin-4/R-Cadherin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cadherin‑4/R‑Cadherin
  His21-Ala734 (Lys347Trp)
  Accession # P55283
• Specificity: Detects human Cadherin‑4/R‑Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) N-Cadherin is observed and less than 1% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-12, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, and rhVE-Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDH4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-4/R-Cadherin Antibody

• CAD4
• cadherin 4, type 1, preproprotein
• cadherin 4, type 1, R-cadherin (retinal)
• Cadherin4
• Cadherin-4
• CDH4
• FLJ22202
• FLJ40547
• MGC126700
• MGC138355
• RCAD
• R-CAD
• R-Cadherin
• Retinal cadherin

Background

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin-4, also known as R-Cadherin, is a classical cadherin of 120‑140 kDa (3, 4). Human Cadherin-4 is synthesized as a 916 amino acid (aa) type I transmembrane glycoprotein that contains a 20 aa signal peptide, a 149 aa prosequence, a 565 aa extracellular region (EC), a 22 aa transmembrane segment, and a 160 aa cytoplasmic domain (5, 6). There are five EC cadherin domains that are approximately 110 aa in length. This pattern is consistent with classical cadherin family molecules that are modular in their extracellular region and mediate calcium-dependent cell-cell adhesion through their Ca⁺⁺-binding repeats (2). One potential Cadherin-4 splice variant involves the preprosegment and shows 32 aa substitution for the N-terminal 124 amino of the full-length precursor (7). The extracellular region of human Cadherin-4 is 96% aa identical to mouse Cadherin-4 extracellular region (3). Cadherin-4 is expressed in vascular smooth muscle (8), pancreatic beta -cells (9), thyroid follicular cells (10), bone marrow Lin^- HSCs (11), sensory neurons of the dorsal root ganglia (12), and, possibly, astrocytes and endothelium of the retina (13). As a classic cadherin, Cadherin-4 will form both homodimers and heterodimers with N-cadherin (4, 14). These complexes translate into adhesion multimers in cis‑ and trans-configurations. Such structures serve to both unite adjacent cells, and provide guidance for migrating cells/processes (13). Additionally, R-Cadherin is associated with cell quiescence, as a loss of cell Cadherin-4 expression is correlated with cell proliferation (8). Finally, R-Cadherin is reported to bind to KLRG1 (killer cell lectin-like receptor G1). This inactivates NK cell cytotoxicity, and provides protection for R-Cadherin expressing cells (15).

1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
3. Matsunami, H. et al. (1993) J. Cell Sci. 106:401.
4. Shan, W-S. et al. (2000) J. Cell Biol. 148:579.
5. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
6. Suzuki, S. et al. (1991) Cell Regul. 2:261.
7. GenBank Accession # BAC03677.
8. Slater, S.C. et al. (2004) Arterioscler. Thromb. Vasc. Biol. 24:1204.
9. Hutton, J.C. et al. (1993) Mol. Endocrinol. 7:1151.
10. Fagman, H. et al. (2003) Endocrinology 144:3618.
11. Dorrell, M.I. et al. (2004) Blood 103:3420.
12. Shibuya, Y. et al. (2005) Kobe J. Med. Sci. 51:35.
13. Dorrell, M.I. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:3500.
14. Murase, S. et al. (2000) Biochem. Biophys. Res. Commun. 276:1191.
15. Ito, M. et al. (2006) J. Exp. Med. 203:289.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH4
• Uniprot:
  • P55283()